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TUG antibody

Anti-TUG antibody used in IHC (Paraffin sections) (IHC-P). GTX33023
Anti-TUG antibody used in IHC (Paraffin sections) (IHC-P). GTX33023
Anti-TUG antibody used in Immunoprecipitation (IP). GTX33023
Anti-TUG antibody used in Western Blot (WB). GTX33023
Anti-TUG antibody used in IHC (Paraffin sections) (IHC-P). GTX33023
Anti-TUG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33023
Anti-TUG antibody used in IHC (Paraffin sections) (IHC-P). GTX33023
Anti-TUG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33023
Anti-TUG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33023

Cat No. GTX33023

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
IP 1:50-1:100
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 284-553 of human ASPSCR1 (NP_076988.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ASPSCR1 tether for SLC2A4, UBX domain containing , ASPCR1 , ASPL , ASPS , RCC17 , TUG , UBXD9 , UBXN9

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene contains a UBX domain and interacts with glucose transporter type 4 (GLUT4). This protein is a tether, which sequesters the GLUT4 in intracellular vesicles in muscle and fat cells in the absence of insulin, and redistributes the GLUT4 to the plasma membrane within minutes of insulin stimulation. Translocation t(X;17)(p11;q25) of this gene with transcription factor TFE3 gene results in a ASPSCR1-TFE3 fusion protein in alveolar soft part sarcoma and in renal cell carcinomas. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-TUG antibody used in IHC (Paraffin sections) (IHC-P). GTX33023

GTX33023 IHC-P Image

IHC-P analysis of human kidney cancer tissue using GTX33023 TUG antibody.
Dilution : 1:100

Anti-TUG antibody used in IHC (Paraffin sections) (IHC-P). GTX33023

GTX33023 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX33023 TUG antibody.
Dilution : 1:100

Anti-TUG antibody used in Immunoprecipitation (IP). GTX33023

GTX33023 IP Image

IP analysis of A431 cells using GTX33023 TUG antibody.
IP reaction : 200μg extracts / 3μg antibody antibody
Dilution : 1:1000

Anti-TUG antibody used in Western Blot (WB). GTX33023

GTX33023 WB Image

WB analysis of various sample lysates using GTX33023 TUG antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-TUG antibody used in IHC (Paraffin sections) (IHC-P). GTX33023

GTX33023 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX33023 TUG antibody.
Dilution : 1:100

Anti-TUG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33023

GTX33023 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX33023 TUG antibody.
Blue : DAPI
Dilution : 1:100

Anti-TUG antibody used in IHC (Paraffin sections) (IHC-P). GTX33023

GTX33023 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX33023 TUG antibody.
Dilution : 1:100

Anti-TUG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33023

GTX33023 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33023 TUG antibody.
Blue : DAPI
Dilution : 1:100

Anti-TUG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33023

GTX33023 ICC/IF Image

ICC/IF analysis of PC-12 cells using GTX33023 TUG antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf