Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TULP1 antibody [C1C3] (GTX103979) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using TULP1(GTX103979) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HeLa, using TULP1(GTX103979) antibody at 1:500 dilution.