APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Rat, Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human TWF2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885383
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
twinfilin actin binding protein 2 , A6RP , A6r , MSTP011 , PTK9L
Cellular Localization
Cytoplasm , cytoskeleton , perinuclear region
Background
The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX115626 IHC-P Image
TWF2 antibody detects TWF2 protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. TWF2 antibody (GTX115626) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX115626 WB Image
TWF2 antibody detects TWF2 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 10 % SDS-PAGE TWF2 antibody (GTX115626) dilution: 1:500
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REFERENCE
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REVIEW
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