GeneTex
United States (US)

TXNDC4 antibody [C1C3]

GTX106636_39974_IHC_19011506_412.jpg
GTX106636_WB_19011506_629.jpg

Cat No. GTX106636

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TXNDC4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

KIAA0573 antibody, PDIA10 antibody, TXNDC4 antibody, ERP44 antibody, ER protein 44 antibody, endoplasmic reticulum resident protein 44 antibody, thioredoxin domain-containing protein 4 antibody, "protein disulfide isomerase family A, member 10 antibody", endoplasmic reticulum resident protein 44 kDa antibody, thioredoxin domain containing 4 (endoplasmic reticulum) antibody, endoplasmic reticulum protein 44 antibody

Cellular Localization

Endoplasmic reticulum lumen

Background

Mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif. Inhibits the calcium channel activity of ITPR1. May have a role in the control of oxidative protein folding in the endoplasmic reticulum. Required to retain ERO1L and ERO1LB in the endoplasmic reticulum.

Database

DATA IMAGES
GTX106636_39974_IHC_19011506_412.jpg

GTX106636 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using TXNDC4(GTX106636) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106636_WB_19011506_629.jpg

GTX106636 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:H1299
10% SDS PAGE
GTX106636 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169