APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-1μg/ml |
| Assay dependent |
| 3.75μg/ml |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX13820-PEP).
Product Note
This antibody is expected to be able to recognise both reported human isoforms, as represented by NP_110437.2; NP_001139021.1.
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-SLHRFVLSQAKDEL, from the C Terminus of the protein sequence according to NP_110437.2; NP_001139021.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
thioredoxin domain containing 5 , ENDOPDI , ERP46 , HCC-2 , HCC2 , PDIA15 , STRF8 , UNQ364
Background
This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal endoplasmic reticulum (ER)-signal sequence, three catalytically active thioredoxin domains and a C-terminal ER-retention sequence. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring upstream BLOC1S5 gene. [provided by RefSeq, Dec 2016]
Database
Research Area
DATA IMAGES
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GTX13820 IHC-P Image
IHC-P analysis of human small intestine using GTX13820 TXNDC5 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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GTX13820 ICC/IF Image
ICC/IF analysis of PFA-fixed U2OS cells using GTX13820 TXNDC5 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control: Unimmunized goat IgG (10μg/ml)
Permeabilization : 0.15% Triton X-100
Dilution : 10μg/ml
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GTX13820 FACS Image
FACS analysis of PFA-fixed HeLa cells using GTX13820 TXNDC5 antibody, C-term.
Blue line: Primary antibody
Black line : IgG control
Permeabilization : 0.5% Triton X-100
Dilution : 10μg/ml
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GTX13820 WB Image
WB analysis of various sample lysates using GTX13820 TXNDC5 antibody, C-term.
A: HEK293
B: A549
C: HeLa
D: HepG2
Loading : 35 μg
Dilution : 0.1μg/ml
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GTX13820 ICC/IF Image
ICC/IF analysis of PFA-fixed HeLa cells using GTX13820 TXNDC5 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control: Unimmunized goat IgG (10μg/ml)
Permeabilization : 0.15% Triton X-100
Dilution : 10μg/ml
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REFERENCE
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REVIEW
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