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TXNDC5 antibody

KO/KD
Anti-TXNDC5 antibody used in Western Blot (WB). GTX106914
Anti-TXNDC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX106914
Anti-TXNDC5 antibody used in Western Blot (WB). GTX106914
Anti-TXNDC5 antibody used in Western Blot (WB). GTX106914

Cat. No. GTX106914

Reference 4
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TXNDC5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

293T , HeLa , HepG2 , Huh-7

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TXNDC5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170188

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thioredoxin domain containing 5 , ENDOPDI , ERP46 , HCC-2 , HCC2 , PDIA15 , STRF8 , UNQ364

Cellular Localization

Endoplasmic reticulum lumen

Background

This gene encodes a protein-disulfide isomerase. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TXNDC5 antibody used in Western Blot (WB). GTX106914

GTX106914 WB Image

KO/KD

Wild-type (WT) and TXNDC5 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TXNDC5 antibody (GTX106914) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TXNDC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX106914

GTX106914 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using TXNDC5(GTX106914) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TXNDC5 antibody used in Western Blot (WB). GTX106914

GTX106914 WB Image

TXNDC5 antibody detects TXNDC5 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TXNDC5 antibody (GTX106914) diluted by 1:1000.

Anti-TXNDC5 antibody used in Western Blot (WB). GTX106914

GTX106914 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: HeLa
C: HepG2
10% SDS PAGE
GTX106914 diluted at 1:1000

REFERENCE

Application Reference

Fujii S et al. Proc Natl Acad Sci U S A 2023; 120 (22) : e2216857120 Redox states in the endoplasmic reticulum directly regulate the activity of calcium channel, inositol 1,4,5-trisphosphate receptors.
Safe S et al. Mol Cancer Res 2019; (Epub) INHIBITION OF NR4A1 PROMOTES ROS ACCUMULATION AND IL-24 - DEPENDENT GROWTH ARREST IN RHABDOMYOSARCOMA.
Hedrick E et al. Endocr Relat Cancer 2015; 22 (5) : 831-40 Nuclear receptor 4A1 as a drug target for breast cancer chemotherapy.
Application : WB
Reactivity : Human
Lee SO et al. Mol Cancer Res 2014; 12 (4) : 527-538 The orphan nuclear receptor NR4A1 (Nur77) regulates oxidative and endoplasmic reticulum stress in pancreatic cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

TXNDC5 antibody Cat. No. GTX106914

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-TXNDC5 antibody used in Western Blot (WB). GTX106914
Date : Anonymous submitted on 02-Nov-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T, HeLa cells
Amount used : 10 μg
Blocking : Blocking One, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169