Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TYK2 antibody [C1C3] (GTX103938) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with TYK2 antibody [C1C3] (GTX103938) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TYK2(GTX103938) antibody at 1:200 dilution.