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TYW1 antibody

Anti-TYW1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116310
Anti-TYW1 antibody used in Western Blot (WB). GTX116310

Cat. No. GTX116310

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TYW1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TYW1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618981

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tRNA-yW synthesizing protein 1 homolog , RSAFD1 , TYW1A , YPL207W

Background

Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW1 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-TYW1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116310

GTX116310 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using TYW1(GTX116310) antibody at 1:200 dilution.

Anti-TYW1 antibody used in Western Blot (WB). GTX116310

GTX116310 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX116310 diluted at 1:10000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169