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TZFP antibody

Anti-TZFP antibody used in Western Blot (WB). GTX115832
Anti-TZFP antibody used in Western Blot (WB). GTX115832

Cat. No. GTX115832

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TZFP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

ZBTB32 transfected 293T , 293T

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TZFP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169734

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger and BTB domain containing 32 , FAXF , FAZF , Rog , TZFP , ZNF538

Cellular Localization

Nucleus

Background

DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T cells.

Database

Research Area

DATA IMAGES

Anti-TZFP antibody used in Western Blot (WB). GTX115832

GTX115832 WB Image

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: ZBTB32 transfected 293T lysates
10% SDS PAGE
GTX115832 diluted at 1:5000

Anti-TZFP antibody used in Western Blot (WB). GTX115832

GTX115832 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX115832 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169