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Talin-1 antibody [97H6]

Anti-Talin-1 antibody [97H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX38972
Anti-Talin-1 antibody [97H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX38972

Cat. No. GTX38972

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 97H6

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μg ($339)
See all Talin-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1/25-1/500
IP Assay dependent

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Not tested in other applications.

Calculated MW

270 kDa. ( Note )

Product Note

This antibody recognizes an epitope within the region of amino acids 482-655 of humanTalin-1.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

his-tagged polypeptide containing residues 489–911 of mouse talin1.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Talin 1 , Tln , Tln1

Background

This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq, Feb 2009]

Research Area

DATA IMAGES

Anti-Talin-1 antibody [97H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX38972

GTX38972 ICC/IF Image

ICC/IF analysis of mouse NIH3T3, undifferentiated C2C12 cells and mouse embryo fibroblasts (MEF) using GTX38972 Talin-1 antibody. Cells were fixed and permeabilised using methanol.

Anti-Talin-1 antibody [97H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX38972

GTX38972 ICC/IF Image

Murine embryonic fibroblast stained with Mouse anti talin 1 antibody, clone 97H6 (GTX38972) and Mouse anti talin-2 antibody, clone 68E7 by ICC/IF demonstrating partial overlapping expression.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339