GeneTex
United States (US)

Talin-1 antibody [97H6]

GTX38973_5502_ICCIF_18121410_174.jpg
GTX38973_5503_ICCIF_18121410_795.jpg

Cat No. GTX38973

Host Mouse
Clonality Monoclonal
Clone Name 97H6
Isotype IgG1
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1/25-1/100
IP Assay dependent

Note :

ICC/IF
Methanol treatment is required prior to antibody staining.

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Not tested in other applications.

Calculated MW

270 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes an epitope within the region of amino acids 482-655 of humanTalin-1.
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

his-tagged polypeptide containing residues 489–911 of mouse talin1.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Talin 1,Tln,Tln1

Background

This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq, Feb 2009]

Research Area

DATA IMAGES
GTX38973_5502_ICCIF_18121410_174.jpg

GTX38973 ICC/IF Image

Murine embryonic fibroblast stained with Mouse anti talin 1 antibody, clone 97H6 (GTX38973) and Mouse anti talin-2 antibody, clone 68E7 by immunofluorescence demonstrating partial overlapping expression.

GTX38973_5503_ICCIF_18121410_795.jpg

GTX38973 ICC/IF Image

ICC/IF analysis of mouse NIH3T3, undifferentiated C2C12 cells and mouse embryo fibroblasts (MEF) using GTX38973 Talin-1 antibody. Cells were fixed and permeabilised using methanol.

Package List Price ($)
$ 349