APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12
Predict Reactivity
Rat, Zebrafish, Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2.09 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Talin-1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1952268
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
talin 1 , ILWEQ , TLN , talin-1
Cellular Localization
Cell projection , ruffle membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , cytoskeleton
Background
This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Talin-1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102215](/templates/default/js/lightbox/images/loading.gif "Anti-Talin-1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102215")
|
GTX102215 IHC-P Image
Talin 1 antibody [C3], C-term detects TLN1 protein at cytosol on H357 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H357 xenograft.
Talin 1 antibody [C3], C-term (GTX102215) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
|
GTX102215 WB Image
Talin 1 antibody detects Talin 1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Talin 1 antibody (GTX102215) diluted by 1:500.
|
|
|
GTX102215 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Talin-1 antibody [C3], C-term (GTX102215) diluted at 1:500.
|
REFERENCE
There are currently no references for Talin-1 antibody [C3], C-term (GTX102215). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Talin-1 antibody [C3], C-term (GTX102215). Be the first to share your experience with this product.
![Anti-Talin-1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102215](/upload/website/prouct_img/small/GTX102215/GTX102215_39911_IHC_w_23060100_405.webp "Anti-Talin-1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102215")
![Anti-Talin-1 antibody [C3], C-term used in Western Blot (WB). GTX102215](/upload/website/prouct_img/small/GTX102215/GTX102215_41801_20141031_WB_w_23060100_786.webp "Anti-Talin-1 antibody [C3], C-term used in Western Blot (WB). GTX102215")
![Anti-Talin-1 antibody [C3], C-term used in Western Blot (WB). GTX102215](/upload/website/prouct_img/small/GTX102215/GTX102215_39911_20161027_WB_M_w_23060100_397.webp "Anti-Talin-1 antibody [C3], C-term used in Western Blot (WB). GTX102215")
![Anti-Talin-1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102215](/upload/website/prouct_img/normal/GTX102215/GTX102215_39911_IHC_w_23060100_405.webp "Anti-Talin-1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102215")
![Anti-Talin-1 antibody [C3], C-term used in Western Blot (WB). GTX102215](/upload/website/prouct_img/normal/GTX102215/GTX102215_41801_20141031_WB_w_23060100_786.webp "Anti-Talin-1 antibody [C3], C-term used in Western Blot (WB). GTX102215")
![Anti-Talin-1 antibody [C3], C-term used in Western Blot (WB). GTX102215](/upload/website/prouct_img/normal/GTX102215/GTX102215_39911_20161027_WB_M_w_23060100_397.webp "Anti-Talin-1 antibody [C3], C-term used in Western Blot (WB). GTX102215")