GeneTex
United States (US)

Talin-1 antibody [C3], C-term

GTX102215_39911_20141031_WB_19011506_389.jpg
GTX102215_39911_IHC_19011506_249.jpg
GTX102215_39911_20161027_WB_M_19011506_291.jpg

Cat No. GTX102215

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

270 kDa. ( Note )

Positive Control

Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , A549 , H1299 , HCT116

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Talin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ILWEQ antibody, KIAA1027 antibody, TLN antibody, TLN1 antibody, talin-1 antibody, talin 1 antibody

Cellular Localization

Cell projection , ruffle membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , cytoskeleton

Background

This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102215_39911_20141031_WB_19011506_389.jpg

GTX102215 WB Image

Talin 1 antibody detects Talin 1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Talin 1 antibody (GTX102215) diluted by 1:500.

GTX102215_39911_IHC_19011506_249.jpg

GTX102215 IHC-P Image

Talin 1 antibody [C3], C-term detects TLN1 protein at cytosol on H357 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H357 xenograft.
Talin 1 antibody [C3], C-term (GTX102215) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102215_39911_20161027_WB_M_19011506_291.jpg

GTX102215 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Talin-1 antibody [C3], C-term (GTX102215) diluted at 1:500.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169