GeneTex
United States (US)

Talin-1 antibody, Internal

GTX88357_2402_WB_18121411_420.jpg

Cat No. GTX88357

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

270 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88357-PEP).

Specificity/Sensitivity

This antibody is not expected to cross-react with the highly similar protein Talin 2.

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-QEALHTQMLTAVQE, from the internal region of the protein sequence according to NP_006280.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TLN1 Antibody , ILWEQ Antibody , TLN Antibody

Cellular Localization

Colocalizes with LAYN at the membrane ruffles.

Background

This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq, Feb 2009]
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Database

Research Area

DATA IMAGES
GTX88357_2402_WB_18121411_420.jpg

GTX88357 WB Image

WB analysis of human peripheral mononucleocytes lysate using GTX88357 Talin-1 antibody, Internal.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

Reference