PRODUCT
Summary
Talin antibody recognizes the cytoskeletal component talin, a ~270 kDa protein involved in cell mechanotransduction that links the cytoskeleton to integrins. It forms an antiparallel homodimer and interacts with vinculin, actin, and integrins. Talin is incorporated very early in the cell-extracellular matrix (ECM) adhesion process.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.54 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Talin-1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2887318
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
talin 1 , ILWEQ , TLN , talin-1
Cellular Localization
Cytoplasm, cytoskeleton
Background
This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq, Feb 2009]
Database
Research Area
DATA IMAGES
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GTX134635 IHC-P Image
Talin-1 antibody detects Talin-1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.Talin-1 stained by Talin-1 antibody (GTX134635) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX134635 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Talin-1 antibody (GTX134635) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX134635 ICC/IF Image
Talin-1 antibody detects Talin-1 protein at cytoplasm and focal adhesion by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Talin-1 stained by Talin-1 antibody (GTX134635) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm.
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REFERENCE
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REVIEW
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