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Talin-2 antibody [53.8]

Anti-Talin-2 antibody [53.8] used in Western Blot (WB). GTX38971
Anti-Talin-2 antibody [53.8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX38971
Anti-Talin-2 antibody [53.8] used in Western Blot (WB). GTX38971

Cat. No. GTX38971

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 53.8

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
200 μg ($469)
See all Talin-2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1/25-1/100
IP Assay dependent
Not tested in other applications.

Calculated MW

254 kDa. ( Note )

Product Note

This antibody recognizes an epitope within the range of amino acids 2470-2500.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant Mouse talin-2 (aa2300-2542).

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Talin 2 , 5730421P04Rik , Ai507121 , Ai787438 , Al118320 , Mkiaa0320 , Tln2

Background

This gene encodes a protein related to talin 1, a cytoskeletal protein that plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. This protein has a different pattern of expression compared to talin 1 but, like talin 1, is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton. [provided by RefSeq, Jul 2008]

Research Area

DATA IMAGES

Anti-Talin-2 antibody [53.8] used in Western Blot (WB). GTX38971

GTX38971 WB Image

WB analysis of anti talin antibody binding to recombinant talin proteins showing specificity of Mouse anti Human talin-2 antibody, clone 53.8 (GTX38971) for talin-2, not recognizing recombinant human talin-1. In contrast Mouse anti talin-1 antibody, clone 97H6 (GTX38973) does not recognize recombinant human talin-2. Clone 8D4, recognizing both talin isoforms.

Anti-Talin-2 antibody [53.8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX38971

GTX38971 ICC/IF Image

ICC/IF analysis of isolated talin-1 null cells using GTX38971 Talin-2 antibody. Cells were fixed in paraformaldehyde and permeablized with triton X-100.

Anti-Talin-2 antibody [53.8] used in Western Blot (WB). GTX38971

GTX38971 WB Image

WB analysis of talin-1 and talin-2 expression in murine tissue lysates using Mouse anti Human talin-2 antibody, clone 53.8 (GTX38971) and Mouse anti Human talin-1 antibody, clone 97H6 (GTX38973). (B) brain, (H) heart, (S) spleen, (K) kidney, (Lu) lung, Li (liver), (M) muscle.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 469