GeneTex
United States (US)

Tankyrase 2 antibody [N1], N-term

GTX104638_39820_IFA_19011506_147.jpg
GTX104638_WB_19011506_935.jpg

Cat No. GTX104638

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2

Predict Reactivity

Mouse(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Tankyrase 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PARP-5b antibody, PARP-5c antibody, PARP5B antibody, PARP5C antibody, TANK2 antibody, TNKL antibody, pART6 antibody, TNKS2 antibody, TNKS-2 antibody, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 antibody, tankyrase-related protein antibody, tankyrase II antibody, tankyrase-like protein antibody, tankyrase 2 antibody, tankyrase-2 antibody, poly [ADP-ribose] polymerase 5B antibody, "tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 antibody"

Cellular Localization

Cytoplasm , Golgi apparatus membrane; Peripheral membrane protein , Nucleus , Telomere (Potential)

Background

May regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. Has PARP activity and can modify TRF1, and thereby contribute to the regulation of telomere length.

Database

Research Area

DATA IMAGES
GTX104638_39820_IFA_19011506_147.jpg

GTX104638 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Tankyrase 2(GTX104638) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX104638_WB_19011506_935.jpg

GTX104638 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
B: HeLa
C: HepG2 (GTX27900)
7.5% SDS PAGE
GTX104638 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169