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Tankyrase 2 antibody [N1], N-term

Anti-Tankyrase 2 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104638
Anti-Tankyrase 2 antibody [N1], N-term used in Western Blot (WB). GTX104638

Cat No. GTX104638

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2

Predict Reactivity

Mouse(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Tankyrase 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tankyrase 2 , ARTD6 , PARP-5b , PARP-5c , PARP5B , PARP5C , TANK2 , TNKL , pART6

Cellular Localization

Cytoplasm , Golgi apparatus membrane; Peripheral membrane protein , Nucleus , Telomere (Potential)

Background

May regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. Has PARP activity and can modify TRF1, and thereby contribute to the regulation of telomere length.

Database

Research Area

DATA IMAGES
Anti-Tankyrase 2 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104638

GTX104638 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Tankyrase 2(GTX104638) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Tankyrase 2 antibody [N1], N-term used in Western Blot (WB). GTX104638

GTX104638 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Tankyrase 2 antibody [N1], N-term (GTX104638) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169