GeneTex

  • Country / Location Selection

United States (US)

Tankyrase antibody

Anti-Tankyrase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117417
Anti-Tankyrase antibody used in IHC (Paraffin sections) (IHC-P). GTX117417
Anti-Tankyrase antibody used in Western Blot (WB). GTX117417

Cat. No. GTX117417

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Monkey
Package
100 μl ($399),
25 μl ($169)
See all Tankyrase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

142 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Tankyrase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10630637

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tankyrase , ARTD5 , PARP-5a , PARP5A , PARPL , TIN1 , TINF1 , TNKS1 , pART5

Cellular Localization

Cytoplasm , Golgi apparatus membrane; Peripheral membrane protein , Nucleus , nuclear pore complex , Telomere (Potential)

Database

Research Area

DATA IMAGES

Anti-Tankyrase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117417

GTX117417 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using Tankyrase(GTX117417) antibody at 1:200 dilution.

Anti-Tankyrase antibody used in IHC (Paraffin sections) (IHC-P). GTX117417

GTX117417 IHC-P Image

Tankyrase antibody detects TNKS protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
Tankyrase antibody (GTX117417) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Tankyrase antibody used in Western Blot (WB). GTX117417

GTX117417 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
5% SDS PAGE
GTX117417 diluted at 1:1000

REFERENCE

Application Reference

Salom? Vilchez Larrea et al. PeerJ 2021; 9 : e11442 First body of evidence suggesting a role of a tankyrase-binding motif (TBM) of vinculin (VCL) in epithelial cells.
Application : WB, ICC/IF
Reactivity : Monkey
Submit a Reference

REVIEW

There are currently no reviews for Tankyrase antibody (GTX117417). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169