IHC-P: Use at a dilution of 1/50. Prolonged fixation in buffered formalin can destroy the epitope. IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
There are two major classes of heat stable microtubule associated proteins (MAPS): MAP 2 (280 kD), and Tau (55-65 kD). Both classes of heat stable MAPS have a role in the regulation of microtubule polymerization in cells. The antibody reacts on immunoblots with Tau proteins and with Tau from Alzheimer paired helical filaments. The antibody does not recognize MAP 1, MAP 2, MAP 5 or tubulin. These antibodies bind both phosphorylated and non-phosphorylated Tau proteins.
This product does not react with Mouse,Rat.
20 mM tris-borate, 150 mM sodium chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, and 0.1% sodium azide, pH 7.5.
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Purified bovine microtuble associated proteins (MAPS)
Tissue culture supernatant
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
MTBT2, MAPTL, P10636, 281296, FTDP-17, MSTD, TAU, FLJ31424, MTBT1, MGC134287, 157140, DDPAC, MAPT, 4137, FTDP17, PPND, FTDP 17
Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
Brain tissues from patients with Alzheimer's are characterized by several histopathological lesions such as neurofibrillary tangles and senile plaques. Neurofibrillary tangles are recognized by anti-Tau while senile plaques are recognized by b-amyloid and ubiquitin antibodies. This antibody localizes Tau proteins along microtubules in axons, somata, dendrites, astrocytes, and on ribosomes (polysomes.).