GeneTex
United States (US)

Tau antibody [Tau]

Cat No. GTX28739

Host Mouse
Clonality Monoclonal
Clone Name Tau
Isotype IgG1
Application IHC-P, IHC-Fr
Reactivity Human, Bovine, Monkey
APPLICATION

Application Note

IHC-P: Use at a dilution of 1/50. Prolonged fixation in buffered formalin can destroy the epitope. IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

46 kDa. ( Note )

Specificity/Sensitivity

There are two major classes of heat stable microtubule associated proteins (MAPS): MAP 2 (280 kD), and Tau (55-65 kD). Both classes of heat stable MAPS have a role in the regulation of microtubule polymerization in cells. The antibody reacts on immunoblots with Tau proteins and with Tau from Alzheimer paired helical filaments. The antibody does not recognize MAP 1, MAP 2, MAP 5 or tubulin. These antibodies bind both phosphorylated and non-phosphorylated Tau proteins.
This product does not react with Mouse,Rat.
PROPERTIES

Form

Liquid

Buffer

20 mM tris-borate, 150 mM sodium chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, and 0.1% sodium azide, pH 7.5.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Antigen Species

Bovine

Immunogen

Purified bovine microtuble associated proteins (MAPS)

Purification

Tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MTBT2, MAPTL, P10636, 281296, FTDP-17, MSTD, TAU, FLJ31424, MTBT1, MGC134287, 157140, DDPAC, MAPT, 4137, FTDP17, PPND, FTDP 17

Cellular Localization

Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.

Background

Brain tissues from patients with Alzheimer's are characterized by several histopathological lesions such as neurofibrillary tangles and senile plaques. Neurofibrillary tangles are recognized by anti-Tau while senile plaques are recognized by b-amyloid and ubiquitin antibodies. This antibody localizes Tau proteins along microtubules in axons, somata, dendrites, astrocytes, and on ribosomes (polysomes.).

Database

Research Area

Package List Price ($)
$ 319