GeneTex
United States (US)

Tau antibody

KO/KD Comparison
GTX100866_42914_20180817_WB_shRNA_watermark_19011506_865.jpg
GTX100866_42914_20171103_WB_M_brain_competitor_watermark_19011506_790.jpg
GTX100866_42914_20181109_IHC-P-FL_19011519_556.jpg
GTX100866_40289_IHC_19011506_865.jpg
GTX100866_42235_20170803_WB_M_brain_19011506_316.jpg
GTX100866_42914_20170810_WB_19011506_464.jpg

Cat No. GTX100866

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

SK-N-SH , IMR32 , SK-N-AS , mouse brain

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tau. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DDPAC antibody, FLJ31424 antibody, FTDP-17 antibody, MAPTL antibody, MGC138549 antibody, MSTD antibody, MTBT1 antibody, MTBT2 antibody, PPND antibody, TAU antibody, MAPT antibody, microtubule-associated protein tau, isoform 4 antibody, G protein beta1/gamma2 subunit-interacting factor 1 antibody, microtubule-associated protein tau antibody, neurofibrillary tangle protein antibody, PHF-tau antibody, paired helical filament-tau antibody

Cellular Localization

Cytoplasm , cytosol , Cell membrane; Peripheral membrane protein; Cytoplasmic side , cytoskeleton , Cell projection , axon

Background

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100866_42914_20180817_WB_shRNA_watermark_19011506_865.jpg

GTX100866 WB Image

KO/KD

Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau antibody (GTX100866) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100866_42914_20171103_WB_M_brain_competitor_watermark_19011506_790.jpg

GTX100866 WB Image

Comparison

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with Tau antibody (GTX100866) diluted at 1:2000 and competitor's antibody (CST#4019) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX100866_42914_20181109_IHC-P-FL_19011519_556.jpg

GTX100866 IHC-P Image

Tau antibody detects Tau protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: Tau stained by Tau antibody (GTX100866) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100866_40289_IHC_19011506_865.jpg

GTX100866 IHC-P Image

Tau antibody detects MAPT protein at cytosol on D54MG xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded D54MG xenograft.
Tau antibody (GTX100866) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100866_42235_20170803_WB_M_brain_19011506_316.jpg

GTX100866 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody (GTX100866) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100866_42914_20170810_WB_19011506_464.jpg

GTX100866 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody (GTX100866) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169