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Tau antibody

Anti-Tau antibody used in Western Blot (WB). GTX116044
Anti-Tau antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116044
Anti-Tau antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116044

Cat. No. GTX116044

Reference 3
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Tau products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Dog, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Tau. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038096

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein tau , DDPAC , FTDP-17 , MAPTL , MSTD , MTBT1 , MTBT2 , PPND , PPP1R103 , TAU

Cellular Localization

Cytoplasm , cytosol , Cell membrane; Peripheral membrane protein; Cytoplasmic side , cytoskeleton , Cell projection , axon

Background

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Tau antibody used in Western Blot (WB). GTX116044

GTX116044 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau antibody (GTX116044) diluted at 1:500.

Anti-Tau antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116044

GTX116044 ICC/IF Image

Tau antibody detects Tau protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tau protein stained by Tau antibody (GTX116044) diluted at 1:400.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-Tau antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116044

GTX116044 ICC/IF Image

The data was published in the journal Stem Cells Int in 2019. PMID: 31065279

REFERENCE

Application Reference

Cort?s-Medina LV et al. Stem Cells Int 2019; 2019 : 7627148 Neuronal Transdifferentiation Potential of Human Mesenchymal Stem Cells from Neonatal and Adult Sources by a Small Molecule Cocktail.
Lorena V. Cort?s-Medina et al. Stem Cells International 2019; 2019 : Article ID 7627148 Neuronal Transdifferentiation Potential of Human Mesenchymal Stem Cells from Neonatal and Adult Sources by a Small Molecule Cocktail.
Application : ICC/IF
Reactivity : Human
Chien MH et al. Mol Cancer Ther 2017; 16 (6) : 1102-1113 A Fas Ligand (FasL)-Fused Humanized Antibody Against Tumor-Associated Glycoprotein 72 Selectively Exhibits the Cytotoxic Effect Against Oral Cancer Cells with a Low FasL/Fas Ratio.
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REVIEW

Tau antibody Cat. No. GTX116044

Rating
( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Tau antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116044
Date : Anonymous submitted on 07-Feb-2017
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : iPSC-derived neurons
Blocking : 3% BSA, 25°C, 0.5Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.5% Triton X-100
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : Human iPSC-derived neurons immunostained for Tau and marked with AlexaFluor 555-conjugated secondary antibody. Visualized by confocal microscope at 40X magnification.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169