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United States (US)

Tau antibody

GTX130462_41927_20170503_IFA_R_18121410_427.jpg
GTX130462_41927_20170713_WB_R_brain_18121410_773.jpg
GTX130462_41927_20170622_IHC-Fr_M_18121410_109.jpg
GTX130462_41927_20181005_IHC-P-FL_M_18121410_733.jpg
GTX130462_41927_20170608_IHC-Fr_R_18121410_756.jpg
GTX130462_41927_20150605_WB_M_brain_18121410_546.jpg
GTX130462_41927_20180817_WB_18121410_111.jpg
GTX130462_41927_20181109_IHC-P-FL_19011519_491.jpg

Cat No. GTX130462

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

SH-SY5Y , rat brain , mouse brain

Predict Reactivity

Bovine, Dog, Chicken, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Tau. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Microtubule Associated Protein Tau,Ddpac,Ftdp-17,Maptl,Mstd,Mtbt1,Mtbt2,Ppnd,Ppp1R103,Tau,Mapt

Cellular Localization

Cytoplasm , cytosol , Cell membrane; Peripheral membrane protein; Cytoplasmic side , cytoskeleton , Cell projection , axon

Background

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX130462_41927_20170503_IFA_R_18121410_427.jpg

GTX130462 ICC/IF Image

Tau antibody detects Tau protein at axon by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tau protein stained by Tau antibody (GTX130462) diluted at 1:500.
Red: MAP2, stained by MAP2 antibody [HM-2] (GTX11267) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX130462_41927_20170713_WB_R_brain_18121410_773.jpg

GTX130462 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody (GTX130462) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX130462_41927_20170622_IHC-Fr_M_18121410_109.jpg

GTX130462 IHC-Fr Image

Tau antibody detects Tau protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse cerebellum.
Green: Tau protein stained by Tau antibody (GTX130462) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX130462_41927_20181005_IHC-P-FL_M_18121410_733.jpg

GTX130462 IHC-P Image

Tau antibody detects Tau protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Green: Tau stained by Tau antibody (GTX130462) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX130462_41927_20170608_IHC-Fr_R_18121410_756.jpg

GTX130462 IHC-Fr Image

Tau antibody detects Tau protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Tau protein stained by Tau antibody (GTX130462) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX130462_41927_20150605_WB_M_brain_18121410_546.jpg

GTX130462 WB Image

Tau antibody detects Tau protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with Tau antibody (GTX130462) at a dilution of 1:10000.

GTX130462_41927_20180817_WB_18121410_111.jpg

GTX130462 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody (GTX130462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX130462_41927_20181109_IHC-P-FL_19011519_491.jpg

GTX130462 IHC-P Image

Tau antibody detects Tau protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: Tau stained by Tau antibody (GTX130462) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169