ICC/IF analysis of SH-SY5Y cells using GTX24846 Tau (phospho Ser214) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1 μg/ml in 1% BSA incubated for 3 hours at room temperature

IHC-P analysis of mouse brain tissue using GTX24846 Tau (phospho Ser214) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

IHC-P analysis of human astroglioma tissue using GTX24846 Tau (phospho Ser214) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

WB analysis of samples using GTX24846 Tau (phospho Ser214) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.