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Tau (phospho Ser396) antibody

Orthogonal
Anti-Tau (phospho Ser396) antibody used in Western Blot (WB). GTX24858

Cat. No. GTX24858

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA, IHC, Neutralizing/Inhibition, IHC (Free Floating)

Reactivity

 Human, Mouse, Rat, Bovine
Package
50 μl ($399)
See all Tau products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P Assay dependent
ELISA Assay dependent
IHC Assay dependent
Neutralizing/Inhibition Assay dependent
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Tau that contains serine 396. The sequence is conserved in many species including mouse, rat, rhesus monkey, baboon, cow and goat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380448

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein tau , DDPAC , FTDP-17 , MAPTL , MSTD , MTBT1 , MTBT2 , PPND , PPP1R103 , TAU

Cellular Localization

Cytoplasm, cytosol,Cell membrane,Cytoplasm, cytoskeleton,Cytoplasm,Cell projection, dendrite

Background

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Tau (phospho Ser396) antibody used in Western Blot (WB). GTX24858

GTX24858 WB Image

Orthogonal

WB (peptide competition) analysis of human recombinant Tau treated with GSK-3 beta (1 μg per μg Tau) for 45 minutes (Lane 2-5) using GTX24858 Tau (phospho Ser396) antibody. The data shows that only the immunogen phospho peptide blocks the signal, demonstrating the specificity of the antibody.
The primary antibody was prior incubated with peptide as following :
Lane 1 : No peptide (Non-treated))
Lane 2 : No peptide (GSK-3 beta treated)
Lane 3: The non-phospho peptide corresponding to immunogen
Lane 4: A generic phosphoserine-containing peptide
Lane 5: The phospho peptide immunogen

REFERENCE

Application Reference

Zeng Y.Q. et al. Journal of Functional Foods 2021; 77 : 104324 Gastrodin as a multi-target protective compound reverses learning memory deficits and AD-like pathology in APP/PS1 transgenic mice.
Chang W et al. J Agric Food Chem 2019; 67 (27) : 7684-7693 Protective Effect of Caffeic Acid against Alzheimer's Disease Pathogenesis via Modulating Cerebral Insulin Signaling, β-Amyloid Accumulation, and Synaptic Plasticity in Hyperinsulinemic Rats.
Application : WB
Reactivity : Rat
Dejanovic B et al. Neuron 2018; 100 (6) : 1322-1336.e7 Changes in the Synaptic Proteome in Tauopathy and Rescue of Tau-Induced Synapse Loss by C1q Antibodies.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399