Tau (phospho Ser409) antibody

IHC-P analysis of mouse brain tissue using GTX24861 Tau (phospho Ser409) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

WB (peptide competition) analysis of human recombinant Tau treated with PKA (36 μg per μg Tau) for 1 hour (Lanes 2-5) using GTX24861 Tau (phospho Ser409) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.