APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
| Assay dependent |
|
Note :
Sandwich ELISA
Capture : GTX638855, Detection : GTX638856 or Capture : GTX638856, Detection : GTX638855
The detection antibody requires HRP conjugation. Please contact us for the conjugation service.
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Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Thr181 of human Tau. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
microtubule associated protein tau , DDPAC , FTDP-17 , MAPTL , MSTD , MTBT1 , MTBT2 , PPND , PPP1R103 , TAU
Cellular Localization
Cytoplasm, cytosol,Cell membrane,Cytoplasm, cytoskeleton,Cell projection, axon,Cytoplasm,Cell projection, dendrite
Background
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Tau (phospho Thr181) antibody [HL2498] used in IHC (Paraffin sections) (IHC-P). GTX638856](/templates/default/js/lightbox/images/loading.gif "Anti-Tau (phospho Thr181) antibody [HL2498] used in IHC (Paraffin sections) (IHC-P). GTX638856")
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GTX638856 IHC-P Image
Tau (phospho Thr181) antibody [HL2498] detects Tau (phospho Thr181) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).Sample: Paraffin-embedded mouse brain.Tau (phospho Thr181) stained by Tau (phospho Thr181) antibody [HL2498] (GTX638856) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638856 ELISA Image
Sandwich ELISA detection of Tau peptide and Tau (phospho Thr181) peptide using antibodies as below. Capture: Tau (phospho Thr181) antibody [HL2497] (GTX638855) (5 μg/mL) Detection: HRP-conjugated Tau (phospho Thr181) antibody [HL2498] (GTX638856) (1 μg/mL)Please notice that GTX638856 needs to be conjugated to HRP to function as the detection antibody when paired with GTX638855. Please contact us for custom HRP-conjugated antibody.
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GTX638856 ELISA Image
Sandwich ELISA detection of Tau peptide and Tau (phospho Thr181) peptide using antibodies as below. Capture: Tau (phospho Thr181) antibody [HL2498] (GTX638856) (5 μg/mL) Detection: HRP-conjugated Tau (phospho Thr181) antibody [HL2497] (GTX638855) (1 μg/mL)Please notice that GTX638855 needs to be conjugated to HRP to function as the detection antibody when paired with GTX638856. Please contact us for custom HRP-conjugated antibody.
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GTX638856 WB Image
Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr181) antibody [HL2498] (GTX638856) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638856 WB Image
Untreated (–) and treated (+) Mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr181) antibody [HL2498] (GTX638856) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for Tau (phospho Thr181) antibody [HL2498] (GTX638856). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Tau (phospho Thr181) antibody [HL2498] (GTX638856). Be the first to share your experience with this product.
![Anti-Tau (phospho Thr181) antibody [HL2498] used in IHC (Paraffin sections) (IHC-P). GTX638856](/upload/website/prouct_img/small/GTX638856/GTX638856_T-45110_20230804_IHC-P_M_1_23090619_301.webp "Anti-Tau (phospho Thr181) antibody [HL2498] used in IHC (Paraffin sections) (IHC-P). GTX638856")
![Anti-Tau (phospho Thr181) antibody [HL2498] used in ELISA (ELISA). GTX638856](/upload/website/prouct_img/small/GTX638856/GTX638856_T-45110_20230908_ELISA_PAIR_Multiple_23091319_965.webp "Anti-Tau (phospho Thr181) antibody [HL2498] used in ELISA (ELISA). GTX638856")
![Anti-Tau (phospho Thr181) antibody [HL2498] used in ELISA (ELISA). GTX638856](/upload/website/prouct_img/small/GTX638856/GTX638856_T-45110_20230908_ELISA_PAIR_Multiple_2_23091319_832.webp "Anti-Tau (phospho Thr181) antibody [HL2498] used in ELISA (ELISA). GTX638856")
![Anti-Tau (phospho Thr181) antibody [HL2498] used in Western Blot (WB). GTX638856](/upload/website/prouct_img/small/GTX638856/GTX638856_45166_20230922_WB_treatment_okadaicacid_23100118_413.webp "Anti-Tau (phospho Thr181) antibody [HL2498] used in Western Blot (WB). GTX638856")
![Anti-Tau (phospho Thr181) antibody [HL2498] used in Western Blot (WB). GTX638856](/upload/website/prouct_img/small/GTX638856/GTX638856_T-45110_20230728_WB_M_treatment_CIP_23073119_258.webp "Anti-Tau (phospho Thr181) antibody [HL2498] used in Western Blot (WB). GTX638856")
![Anti-Tau (phospho Thr181) antibody [HL2498] used in IHC (Paraffin sections) (IHC-P). GTX638856](/upload/website/prouct_img/normal/GTX638856/GTX638856_T-45110_20230804_IHC-P_M_1_23090619_301.webp "Anti-Tau (phospho Thr181) antibody [HL2498] used in IHC (Paraffin sections) (IHC-P). GTX638856")
![Anti-Tau (phospho Thr181) antibody [HL2498] used in ELISA (ELISA). GTX638856](/upload/website/prouct_img/normal/GTX638856/GTX638856_T-45110_20230908_ELISA_PAIR_Multiple_23091319_965.webp "Anti-Tau (phospho Thr181) antibody [HL2498] used in ELISA (ELISA). GTX638856")
![Anti-Tau (phospho Thr181) antibody [HL2498] used in ELISA (ELISA). GTX638856](/upload/website/prouct_img/normal/GTX638856/GTX638856_T-45110_20230908_ELISA_PAIR_Multiple_2_23091319_832.webp "Anti-Tau (phospho Thr181) antibody [HL2498] used in ELISA (ELISA). GTX638856")
![Anti-Tau (phospho Thr181) antibody [HL2498] used in Western Blot (WB). GTX638856](/upload/website/prouct_img/normal/GTX638856/GTX638856_45166_20230922_WB_treatment_okadaicacid_23100118_413.webp "Anti-Tau (phospho Thr181) antibody [HL2498] used in Western Blot (WB). GTX638856")
![Anti-Tau (phospho Thr181) antibody [HL2498] used in Western Blot (WB). GTX638856](/upload/website/prouct_img/normal/GTX638856/GTX638856_T-45110_20230728_WB_M_treatment_CIP_23073119_258.webp "Anti-Tau (phospho Thr181) antibody [HL2498] used in Western Blot (WB). GTX638856")