Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr181) antibody [HL2498] (GTX638856) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sandwich ELISA detection of Tau peptide and Tau (phospho Thr181) peptide using antibodies as below.

Capture: Tau (phospho Thr181) antibody [HL2498] (GTX638856) (5 μg/mL)
Detection: HRP-conjugated Tau (phospho Thr181) antibody [HL2497] (GTX638855) (1 μg/mL)
Please notice that GTX638855 needs to be conjugated to HRP to function as the detection antibody when paired with GTX638856. Please contact us for custom HRP-conjugated antibody.

Sandwich ELISA detection of Tau peptide and Tau (phospho Thr181) peptide using antibodies as below.

Capture: Tau (phospho Thr181) antibody [HL2497] (GTX638855) (5 μg/mL)
Detection: HRP-conjugated Tau (phospho Thr181) antibody [HL2498] (GTX638856) (1 μg/mL)
Please notice that GTX638856 needs to be conjugated to HRP to function as the detection antibody when paired with GTX638855. Please contact us for custom HRP-conjugated antibody.

Tau (phospho Thr181) antibody [HL2498] detects Tau (phospho Thr181) protein in the cytoplasm by immunohistochemical analysis in the presence of non phospho control peptide (left) but not in the presence of the antigenic phospho peptide (right).
Sample: Paraffin-embedded mouse brain.
Tau (phospho Thr181) stained by Tau (phospho Thr181) antibody [HL2498] (GTX638856) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Untreated (–) and treated (+) Mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr181) antibody [HL2498] (GTX638856) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.