APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Observed MW
50~80 kDa.
Predict Reactivity
Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.57 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Thr181 of human Tau. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2909880
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
microtubule associated protein tau , DDPAC , FTDP-17 , MAPTL , MSTD , MTBT1 , MTBT2 , PPND , PPP1R103 , TAU
Cellular Localization
Cytoplasm, cytosol,Cell membrane,Cytoplasm, cytoskeleton,Cytoplasm,Cell projection, dendrite
Background
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX135776 IHC-P Image
Tau (phospho Thr181) antibody detects Tau (phospho Thr181) protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Tau (phospho Thr181) stained by Tau (phospho Thr181) antibody (GTX135776) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX135776 WB Image
Untreated (–) and treated (+) Mouse tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr181) antibody (GTX135776) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX135776 WB Image
Untreated (–) and treated (+) SK-N-SH whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr181) antibody (GTX135776) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX135776 IHC-P Image
Tau (phospho Thr181) antibody detects Tau (phospho Thr181) protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Tau (phospho Thr181) stained by Tau (phospho Thr181) antibody (GTX135776) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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