APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Bovine, Dog, Chicken, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Thr217 of human Tau. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2910044
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
microtubule associated protein tau , DDPAC , FTDP-17 , MAPTL , MSTD , MTBT1 , MTBT2 , PPND , PPP1R103 , TAU
Cellular Localization
Cytoplasm, cytosol,Cell membrane,Cytoplasm, cytoskeleton,Cytoplasm,Cell projection, dendrite
Background
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Tau (phospho Thr217) antibody [HL1276] used in Western Blot (WB). GTX636678](/templates/default/js/lightbox/images/loading.gif "Anti-Tau (phospho Thr217) antibody [HL1276] used in Western Blot (WB). GTX636678")
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GTX636678 WB Image
Untreated (–) and treated (+) SK-N-SH whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr217) antibody [HL1276] (GTX636678) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX636678 ICC/IF Image
Tau (phospho Thr217) antibody [HL1276] detects Tau (phospho Thr217) protein at cytoskeleton by immunofluorescent analysis.Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody [HL1276] (GTX636678) diluted at 1:250.Red: Tau, a cytoskeleton marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636678 WB Image
Untreated (–) and treated (+) SK-N-SH whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr217) antibody [HL1276] (GTX636678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636678 IHC-P Image
Tau (phospho Thr217) antibody [HL1276] detects Tau (phospho Thr217) protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody [HL1276] (GTX636678) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636678 ELISA Image
Indirect ELISA analysis was performed by coating the plate with Tau peptide and Tau (phospho Thr217) peptide (1000-15.63 ng/mL). Coated peptides were probed with Tau (phospho Thr217) antibody [HL1276] (GTX636678) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
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GTX636678 ICC/IF Image
Tau (phospho Thr217) antibody [HL1276] detects Tau (phospho Thr217) protein at cytoskeleton by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody [HL1276] (GTX636678) diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for Tau (phospho Thr217) antibody [HL1276] (GTX636678). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Tau (phospho Thr217) antibody [HL1276] (GTX636678). Be the first to share your experience with this product.
![Anti-Tau (phospho Thr217) antibody [HL1276] used in Western Blot (WB). GTX636678](/upload/website/prouct_img/small/GTX636678/GTX636678_44585_20220701_WB_treatment_Okadaicacid_watermark_22070501_632.webp "Anti-Tau (phospho Thr217) antibody [HL1276] used in Western Blot (WB). GTX636678")
![Anti-Tau (phospho Thr217) antibody [HL1276] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636678](/upload/website/prouct_img/small/GTX636678/GTX636678_44585_20200715_ICC_IF_22072519_713.webp "Anti-Tau (phospho Thr217) antibody [HL1276] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636678")
![Anti-Tau (phospho Thr217) antibody [HL1276] used in Western Blot (WB). GTX636678](/upload/website/prouct_img/small/GTX636678/GTX636678_44585_20220610_WB_treatment_okadaicacid_22060523_307.jpg "Anti-Tau (phospho Thr217) antibody [HL1276] used in Western Blot (WB). GTX636678")
![Anti-Tau (phospho Thr217) antibody [HL1276] used in IHC (Paraffin sections) (IHC-P). GTX636678](/upload/website/prouct_img/small/GTX636678/GTX636678_44585_20220311_IHC-P_M_22031423_574.jpg "Anti-Tau (phospho Thr217) antibody [HL1276] used in IHC (Paraffin sections) (IHC-P). GTX636678")
![Anti-Tau (phospho Thr217) antibody [HL1276] used in ELISA (ELISA). GTX636678](/upload/website/prouct_img/small/GTX636678/GTX636678_44585_20220408_ELISA_Indirect_Multiple_22041900_800.jpg "Anti-Tau (phospho Thr217) antibody [HL1276] used in ELISA (ELISA). GTX636678")
![Anti-Tau (phospho Thr217) antibody [HL1276] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636678](/upload/website/prouct_img/small/GTX636678/GTX636678_44585_20221209_ICC_IF_R_22122018_964.webp "Anti-Tau (phospho Thr217) antibody [HL1276] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636678")
![Anti-Tau (phospho Thr217) antibody [HL1276] used in Western Blot (WB). GTX636678](/upload/website/prouct_img/normal/GTX636678/GTX636678_44585_20220701_WB_treatment_Okadaicacid_watermark_22070501_632.webp "Anti-Tau (phospho Thr217) antibody [HL1276] used in Western Blot (WB). GTX636678")
![Anti-Tau (phospho Thr217) antibody [HL1276] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636678](/upload/website/prouct_img/normal/GTX636678/GTX636678_44585_20200715_ICC_IF_22072519_713.webp "Anti-Tau (phospho Thr217) antibody [HL1276] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636678")
![Anti-Tau (phospho Thr217) antibody [HL1276] used in Western Blot (WB). GTX636678](/upload/website/prouct_img/normal/GTX636678/GTX636678_44585_20220610_WB_treatment_okadaicacid_22060523_307.jpg "Anti-Tau (phospho Thr217) antibody [HL1276] used in Western Blot (WB). GTX636678")
![Anti-Tau (phospho Thr217) antibody [HL1276] used in IHC (Paraffin sections) (IHC-P). GTX636678](/upload/website/prouct_img/normal/GTX636678/GTX636678_44585_20220311_IHC-P_M_22031423_574.jpg "Anti-Tau (phospho Thr217) antibody [HL1276] used in IHC (Paraffin sections) (IHC-P). GTX636678")
![Anti-Tau (phospho Thr217) antibody [HL1276] used in ELISA (ELISA). GTX636678](/upload/website/prouct_img/normal/GTX636678/GTX636678_44585_20220408_ELISA_Indirect_Multiple_22041900_800.jpg "Anti-Tau (phospho Thr217) antibody [HL1276] used in ELISA (ELISA). GTX636678")
![Anti-Tau (phospho Thr217) antibody [HL1276] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636678](/upload/website/prouct_img/normal/GTX636678/GTX636678_44585_20221209_ICC_IF_R_22122018_964.webp "Anti-Tau (phospho Thr217) antibody [HL1276] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636678")