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Tau (phospho Thr217) antibody [HL1276]

Recombinant antibody Orthogonal
Anti-Tau (phospho Thr217) antibody [HL1276] used in ELISA (ELISA). GTX636678
Anti-Tau (phospho Thr217) antibody [HL1276] used in IHC (Paraffin sections) (IHC-P). GTX636678
Anti-Tau (phospho Thr217) antibody [HL1276] used in Western Blot (WB). GTX636678
Anti-Tau (phospho Thr217) antibody [HL1276] used in Western Blot (WB). GTX636678
Anti-Tau (phospho Thr217) antibody [HL1276] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636678

Cat No. GTX636678

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1276

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)
See all Tau products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr217 of human Tau. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910044

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

microtubule associated protein tau , DDPAC , FTDP-17 , MAPTL , MSTD , MTBT1 , MTBT2 , PPND , PPP1R103 , TAU

Cellular Localization

Cytoplasm, cytosol,Cell membrane,Cytoplasm, cytoskeleton,Cytoplasm,Cell projection, dendrite

Background

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Tau (phospho Thr217) antibody [HL1276] used in ELISA (ELISA). GTX636678

GTX636678 ELISA Image

Indirect ELISA analysis was performed by coating the plate with Tau peptide and Tau (phospho Thr217) peptide (1000-15.63 ng/mL). Coated peptides were probed with Tau (phospho Thr217) antibody [HL1276] (GTX636678) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Anti-Tau (phospho Thr217) antibody [HL1276] used in IHC (Paraffin sections) (IHC-P). GTX636678

GTX636678 IHC-P Image

Tau (phospho Thr217) antibody [HL1276] detects Tau (phospho Thr217) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody [HL1276] (GTX636678) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Tau (phospho Thr217) antibody [HL1276] used in Western Blot (WB). GTX636678

GTX636678 WB Image

Untreated (–) and treated (+) SK-N-SH whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr217) antibody [HL1276] (GTX636678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Tau (phospho Thr217) antibody [HL1276] used in Western Blot (WB). GTX636678

GTX636678 WB Image

Orthogonal

Untreated (–) and treated (+) SK-N-SH whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr217) antibody [HL1276] (GTX636678) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Tau (phospho Thr217) antibody [HL1276] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636678

GTX636678 ICC/IF Image

Tau (phospho Thr217) antibody [HL1276] detects Tau (phospho Thr217) protein at cytoskeleton by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody [HL1276] (GTX636678) diluted at 1:250.
Red: Tau, a cytoskeleton marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 379
$ 169
Custom Conjugations