Tau (phospho Thr217) antibody [HL1276]

Cat No. GTX636678

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1276
Isotype	IgG
Application	WB, IHC-P, ELISA
Reactivity	Human, Mouse
Package	100 μl ($369), 25 μl ($169)

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Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
IHC-P	Assay dependent
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

79 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Tau (phospho Thr217). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910044

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

microtubule associated protein tau , DDPAC , FTDP-17 , MAPTL , MSTD , MTBT1 , MTBT2 , PPND , PPP1R103 , TAU

Cellular Localization

Cytoplasm, cytosol,Cell membrane,Cytoplasm, cytoskeleton,Cytoplasm,Cell projection, dendrite

Background

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]

Database

Gene ID: 4137 MAPT
UniProt: P10636 MAPT

Research Area

DATA IMAGES

GTX636678 IHC-P Image

Tau (phospho Thr217) antibody [HL1276] detects Tau (phospho Thr217) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody [HL1276] (GTX636678) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636678 WB Image

Untreated (–) and treated (+) SK-N-SH whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr217) antibody [HL1276] (GTX636678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636678 ELISA Image

Indirect ELISA analysis was performed by coating the plate with Tau peptide and Tau (phospho Thr217) peptide (1000-15.63 ng/mL). Coated peptides were probed with Tau (phospho Thr217) antibody [HL1276] (GTX636678) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

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Package

List Price ($)

100 μl

$ 369

25 μl

$ 169

Qty Custom Conjugations