APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Bovine, Dog, Chicken, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.35 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Thr217 of human Tau. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2909879
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
microtubule associated protein tau , DDPAC , FTDP-17 , MAPTL , MSTD , MTBT1 , MTBT2 , PPND , PPP1R103 , TAU
Cellular Localization
Cytoplasm, cytosol,Cell membrane,Cytoplasm, cytoskeleton,Cytoplasm,Cell projection, dendrite
Background
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX135775 IHC-P Image
Tau (phospho Thr217) antibody detects Tau (phospho Thr217) protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody (GTX135775) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX135775 WB Image
Untreated (–) and treated (+) Neuro2A whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr217) antibody (GTX135775) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX135775 ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant partial Tau peptide and Tau (phospho Thr217) peptide (1000-0.003 ng/mL). Coated protein was probed with Tau (phospho Thr217) antibody (GTX135775) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
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REFERENCE
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REVIEW
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