GeneTex
United States (US)

TdT antibody [C1C3]

Orthogonal Overexpression
GTX108697_41577_WB_Fraction_19011507_743.jpg
GTX108697_41577_WB_B_19011507_879.jpg

Cat No. GTX108697

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Jurkat , Jurkat nuclear , human DNTT-transfected 293T

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TdT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TDT antibody, DNTT antibody, terminal addition enzyme antibody, terminal transferase antibody, DNA nucleotidylexotransferase antibody, terminal deoxynucleotidyltransferase antibody, terminal deoxyribonucleotidyltransferase antibody, nucleosidetriphosphate:DNA deoxynucleotidylexotransferase antibody, "deoxynucleotidyltransferase, terminal antibody"

Cellular Localization

Nucleus

Background

This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX108697_41577_WB_Fraction_19011507_743.jpg

GTX108697 WB Image

Orthogonal

TdT antibody [C1C3] detects TdT protein by western blot analysis.
A. 30 μg Jurkat whole cell extract
B. 30 μg Jurkat nuclear extract
7.5 % SDS-PAGE
TdT antibody [C1C3] (GTX108697) dilution: 1:500

GTX108697_41577_WB_B_19011507_879.jpg

GTX108697 WB Image

Overexpression

TdT antibody [C1C3] detects TdT protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of human DNTT-transfected 293T cells
7.5 % SDS-PAGE
TdT antibody [C1C3] (GTX108697) dilution: 1:5000

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf