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TdT antibody [C1C3]

Orthogonal Overexpression
Anti-TdT antibody [C1C3] used in Western Blot (WB). GTX108697
Anti-TdT antibody [C1C3] used in Western Blot (WB). GTX108697

Cat. No. GTX108697

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TdT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

human DNTT-transfected 293T cells , Jurkat nuclear extract

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TdT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950137

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA nucleotidylexotransferase , TDT

Cellular Localization

Nucleus

Background

This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TdT antibody [C1C3] used in Western Blot (WB). GTX108697

GTX108697 WB Image

Orthogonal

TdT antibody [C1C3] detects TdT protein by western blot analysis.
A. 30 μg Jurkat whole cell extract
B. 30 μg Jurkat nuclear extract
7.5 % SDS-PAGE
TdT antibody [C1C3] (GTX108697) dilution: 1:500

Anti-TdT antibody [C1C3] used in Western Blot (WB). GTX108697

GTX108697 WB Image

Overexpression

TdT antibody [C1C3] detects TdT protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of human DNTT-transfected 293T cells
7.5 % SDS-PAGE
TdT antibody [C1C3] (GTX108697) dilution: 1:5000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169