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Tenascin C antibody [C3], C-term

Anti-Tenascin C antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100558
Anti-Tenascin C antibody [C3], C-term used in Western Blot (WB). GTX100558

Cat. No. GTX100558

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Dog
Package
100 μl ($399),
25 μl ($169)
See all Tenascin C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

241 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Tenascin C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241383

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tenascin C , 150-225 , DFNA56 , GMEM , GP , HXB , JI , TN , TN-C

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.

Database

Research Area

DATA IMAGES

Anti-Tenascin C antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100558

GTX100558 IHC-P Image

Tenascin-C antibody [C3], C-term detects Tenascin-C protein at cytoplasm on human uterus vessel by immunohistochemical analysis.
Sample: Paraffin-embedded uterus vessel.
Tenascin-C antibody [C3], C-term (GTX100558) dilution: 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Tenascin C antibody [C3], C-term used in Western Blot (WB). GTX100558

GTX100558 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Tenascin C antibody [C3], C-term (GTX100558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Khazaei S et al. J Tissue Eng Regen Med 2020; 14 (9) : 1227-1235 Improving pulp revascularization outcomes with buccal fat autotransplantation.
Application : IHC-P
Reactivity : Dog
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169