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Tet Repressor antibody

Anti-Tet Repressor antibody used in ELISA (ELISA). GTX131985
Anti-Tet Repressor antibody used in Western Blot (WB). GTX131985

Cat. No. GTX131985

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.16 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length Tet Repressor recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886544

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-Tet Repressor antibody used in ELISA (ELISA). GTX131985

GTX131985 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length Tet Repressor (1000-15.63 ng/mL). Coated protein was probed with Tet Repressor antibody (GTX131985) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-Tet Repressor antibody used in Western Blot (WB). GTX131985

GTX131985 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Tet Repressor antibody (GTX131985) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169