GeneTex

  • Country / Location Selection

United States (US)

Tet Repressor antibody

Anti-Tet Repressor antibody used in Western Blot (WB). GTX70489
Anti-Tet Repressor antibody used in Western Blot (WB). GTX70489
Anti-Tet Repressor antibody used in ELISA (ELISA). GTX70489

Cat. No. GTX70489

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Species independent
Package
100 μl ($399)
See all Tet Repressor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:10000
ELISA 1:1000-1:10000
Not tested in other applications.

Positive Control

Tetracyclin repressor (tetR)

Product Note

The antibody works in any species where the TetR system is being used to regulate gene expression in mammalian cells.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.27 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Reacts with residues 31-49 [AQKLGVEQPTLYWHVKNKR] of the 22 kDa Tet repressor protein. Sequence is 100% conserved in all TetR classes.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_383262

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pSC101\tetR antibody Tet Repressor Protein antibody Tetracycline Repressor Protein antibody

Background

The Tet repressor protein (TetR) regulates transcription of a family of tetracycline resistance determinants in Gram-negative bacteria. The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator.

Research Area

DATA IMAGES

Anti-Tet Repressor antibody used in Western Blot (WB). GTX70489

GTX70489 WB Image

Detection of Tet- Repressor (TetR) using anti-TetR (GTX70489) at a 1:1000 dilution.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Tet Repressor antibody used in Western Blot (WB). GTX70489

GTX70489 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Tetracyclin repressor (tetR) antibody (GTX131984) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Tet Repressor antibody used in ELISA (ELISA). GTX70489

GTX70489 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Tet Repressor protein (162.21-2.53 nM). Coated protein was probed with Tet Repressor antibody (GTX70489) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

REFERENCE

Application Reference

Fang M et al. Cell 2010; 143 (5) : 711-24 The ER UDPase ENTPD5 promotes protein N-glycosylation, the Warburg effect, and proliferation in the PTEN pathway.
Submit a Reference

REVIEW

There are currently no reviews for Tet Repressor antibody (GTX70489). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399