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Tet Repressor antibody

Anti-Tet Repressor antibody used in Western Blot (WB). GTX70489
Anti-Tet Repressor antibody used in Western Blot (WB). GTX70489
Anti-Tet Repressor antibody used in ELISA (ELISA). GTX70489

Cat No. GTX70489

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human
Package
100 μl ($299)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:10000
ELISA 1:1000-1:10000
Not tested in other applications.

Positive Control

Tetracyclin repressor (tetR)

Specificity/Sensitivity

The antibody works in any species where the TetR system is being used to regulate gene expression in mammalian cells.

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.53 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Reacts with residues 31-49 [AQKLGVEQPTLYWHVKNKR] of the 22 kDa Tet repressor protein. Sequence is 100% conserved in all TetR classes.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

pSC101\tetR antibody Tet Repressor Protein antibody Tetracycline Repressor Protein antibody

Background

The Tet repressor protein (TetR) regulates transcription of a family of tetracycline resistance determinants in Gram-negative bacteria. The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator.

Research Area

DATA IMAGES

Anti-Tet Repressor antibody used in Western Blot (WB). GTX70489

GTX70489 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Tetracyclin repressor (GTX70489) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Tet Repressor antibody used in Western Blot (WB). GTX70489

GTX70489 WB Image

Detection of Tet- Repressor (TetR) using anti-TetR (GTX70489) at a 1:1000 dilution.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Tet Repressor antibody used in ELISA (ELISA). GTX70489

GTX70489 ELISA Image

An ELISA plate is coated with 50 μL of Tet Repressor peptide at concentration ranged from 0.01 μg/mL to 3 μg/mL. The coated peptide is detected with Tet Repressor antibody (GTX70489) at 0.5 μg/mL.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 299