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ThPok antibody

Anti-ThPok antibody used in IHC (Paraffin sections) (IHC-P). GTX48861

Cat. No. GTX48861

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
50 μl ($399)
See all ThPok products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:3000
IHC-P 1.25 - 2.5 μg/ml
ELISA 1:2500 - 1:10000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of mouse ThPok protein.

Purification

Antiserum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger and BTB domain containing 7B , Thpok , Zfp67 , c-Krox

Cellular Localization

Nucleus

Background

cKrox (also known as Zfp67 or ThPOK) functions as a transcription regulator that acts as a key modulator of lineage commitment for immature T-cell precursors. cKrox is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. cKrox is a known transcriptional repressor of the collagen COL1A1 and COL1A2 genes and may also function as a repressor of fibronectin and possibly other extracellular matrix genes. cKrox is located within the nucleus and is expressed in multiple tissues and organs including skin, thymus and peripheral T-cells.

Database

Research Area

DATA IMAGES

Anti-ThPok antibody used in IHC (Paraffin sections) (IHC-P). GTX48861

GTX48861 IHC-P Image

Immunohistochemical analysis of paraffin-embedded small intestine tissue, using Ckrox antibody GTX48861 at 1:100 dilution. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399