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Thbs4b antibody

Anti-Thbs4b antibody used in Western Blot (WB). GTX125869
Anti-Thbs4b antibody used in IHC (Whole mount) (IHC-Wm). GTX125869
Anti-Thbs4b antibody used in IHC (Whole mount) (IHC-Wm). GTX125869

Cat. No. GTX125869

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Wm

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Thbs4b products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

zebrafish muscle

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of zebrafish Thbs4b. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885605

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thrombospondin 4b , thbs4 , tsp4 , zgc:111910

Background

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen.

Database

Research Area

DATA IMAGES

Anti-Thbs4b antibody used in Western Blot (WB). GTX125869

GTX125869 WB Image

Sample (30 ug of whole cell lysate)
A: zebrafish muscle
7.5% SDS PAGE
GTX125869 diluted at 1:1000

Anti-Thbs4b antibody used in IHC (Whole mount) (IHC-Wm). GTX125869

GTX125869 IHC-Wm Image

Thbs4b antibody detects Thbs4b protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Thbs4b antibody (GTX125869) dilution: 1:100.
Anti-Thbs4b antibody used in IHC (Whole mount) (IHC-Wm). GTX125869

GTX125869 IHC-Wm Image

Thbs4b antibody detects Thbs4b protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Thbs4b antibody (GTX125869) dilution: 1:100.

REFERENCE

Application Reference

Ghosal R et al. Front Physiol 2023; 14 : 1131075 Embryonic ethanol exposure disrupts craniofacial neuromuscular integration in zebrafish larvae.
Application : IHC-Wm
Reactivity : Zebrafish
McGurk PD et al. PLoS Genet 2017; 13 (12) : e1007112 In vivo zebrafish morphogenesis shows Cyp26b1 promotes tendon condensation and musculoskeletal patterning in the embryonic jaw.
Application : IHC-Wm
Reactivity : Zebrafish
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169