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Thioredoxin 1 protein, His tag (active)

Thioredoxin 1 protein, His tag (active). GTX66886-pro

Cat No. GTX66886-pro

Application

Functional Assay

Species

E. coli
Package
50 μg ($289)

APPLICATION

Application Note

Specific activity is > 70 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

PROPERTIES

Form

Liquid

Buffer

20 mM Tris-HCl buffer (pH 8.0) with 10% glycerol, 1 mM DTT.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml

Region/Sequence

N-terminal His-Tag; Length: 117 a.a. Sequence: MHHHHHHMGS DKIIHLTDDS FDTDVLKADG AILVDFWAEW CGPCKMIAPI LDEIADEYQG KLTVAKLNID QNPGTAPKYG IRGIPTLLLF KNGEVAATKV GALSKGQLKE FLDANLA

Expression System

E.coli

Purity

> 95% by SDS-PAGE.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

thiol reductase thioredoxin , CFNIH1_03935

Database

Research Area

DATA IMAGES

Thioredoxin 1 protein, His tag (active). GTX66886-pro

GTX66886-pro Image

3 μg of GTX66886-pro Thioredoxin 1 protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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REVIEW

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SDS
Glycerol.pdf