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Thioredoxin antibody [N3C3]

Overexpression
Anti-Thioredoxin antibody [N3C3] used in Western Blot (WB). GTX108263
Anti-Thioredoxin antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108263
Anti-Thioredoxin antibody [N3C3] used in Western Blot and Immunoprecipitation (WB IP). GTX108263

Cat. No. GTX108263

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Thioredoxin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

H1299 , HeLaS3

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Thioredoxin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952454

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thioredoxin , TRDX , TRX , TRX1

Cellular Localization

Cytoplasm

Background

Thioredoxin is a 12-kD oxidoreductase enzyme containing a dithiol-disulfide active site. It is ubiquitous and found in many organisms from plants and bacteria to mammals. Multiple in vitro substrates for thioredoxin have been identified, including ribonuclease, choriogonadotropins, coagulation factors, glucocorticoid receptor, and insulin. Reduction of insulin is classically used as an activity test.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Thioredoxin antibody [N3C3] used in Western Blot (WB). GTX108263

GTX108263 WB Image

Sample(30 μg of whole cell lysate)
A:H1299
B:HeLa S3(GTX14654)
12% SDS PAGE
GTX108263 diluted at 1:3000

Anti-Thioredoxin antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108263

GTX108263 ICC/IF Image

Trx antibody [N3C3] detects Trx protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Trx protein stained by Trx antibody [N3C3] (GTX108263) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Thioredoxin antibody [N3C3] used in Western Blot and Immunoprecipitation (WB IP). GTX108263

GTX108263 WB IP Image

Overexpression

The data was published in the journal Biomedicines in 2020. PMID: 32244938

REFERENCE

Application Reference

Nagano N et al. Biomedicines 2020; 8 (3) Attenuation of Hyperoxic Lung Injury in Newborn Thioredoxin-1-Overexpressing Mice through the Suppression of Proinflammatory Cytokine mRNA Expression.
Application : IP
Reactivity : Mouse
Lin WC et al. RSC Adv. 2017; 7 : 21234-21251 Anti-inflammatory activity of Sanghuangporus sanghuang by suppressing the TLR4-mediated PI3K/AKT/mTOR/IKKβ signaling pathway.
Application : WB
Reactivity : Mouse
Ueno H et al. Biol Pharm Bull 2014; 37 (8) : 1352-8 Effect of Selenite on T-Cell Mitogenesis: Contribution of ROS Production and Apoptosis Signal-Regulating Kinase 1.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169