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Thioredoxin antibody

Anti-Thioredoxin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64390
Anti-Thioredoxin antibody used in Western Blot (WB). GTX64390
Anti-Thioredoxin antibody used in Immunoprecipitation (IP). GTX64390
Anti-Thioredoxin antibody used in IHC (Paraffin sections) (IHC-P). GTX64390

Cat. No. GTX64390

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Thioredoxin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 20 to the C-terminus of human TXN (NP_003320.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thioredoxin , TRDX , TRX , TRX1

Cellular Localization

Nucleus,Cytoplasm,Secreted

Background

The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Thioredoxin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64390

GTX64390 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX64390 Thioredoxin antibody.
Blue : DAPI
Dilution : 1:100

Anti-Thioredoxin antibody used in Western Blot (WB). GTX64390

GTX64390 WB Image

WB analysis of various sample lysates using GTX64390 Thioredoxin antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Thioredoxin antibody used in Immunoprecipitation (IP). GTX64390

GTX64390 IP Image

IP analysis of MCF7 cell lysate using GTX64390 Thioredoxin antibody.
Antibody amount : 3μg / 150μg lysate
Dilution : 1:500

Anti-Thioredoxin antibody used in IHC (Paraffin sections) (IHC-P). GTX64390

GTX64390 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX64390 Thioredoxin antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399