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Thrombin Receptor antibody

Anti-Thrombin Receptor antibody used in Flow cytometry (FACS). GTX54827
Anti-Thrombin Receptor antibody used in Western Blot (WB). GTX54827
Anti-Thrombin Receptor antibody used in Western Blot (WB). GTX54827
Anti-Thrombin Receptor antibody used in Live cell imaging (LCI). GTX54827
Anti-Thrombin Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX54827

Cat. No. GTX54827

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, LCI

Reactivity

 Human
Package
50 μl ($639)
See all Thrombin Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
FACS Assay dependent
LCI Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Product Note

We do not recommend use of this product for Mouse,Rat samples.

Predict Reactivity

Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide (C)KNESGLTEYRLVSINK, corresponding to amino acid residues 61-76 (Extracellular, N terminal) of human PAR-1 (Accession P25116).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coagulation factor II thrombin receptor , CF2R , HTR , PAR-1 , PAR1 , TR

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-Thrombin Receptor antibody used in Flow cytometry (FACS). GTX54827

GTX54827 FACS Image

FACS analysis of HL-60 cells using GTX54827 Thrombin Receptor antibody.
Black : Unstained cell
Green : Cell staining with primary antibody
Dilution : 1:20

Anti-Thrombin Receptor antibody used in Western Blot (WB). GTX54827

GTX54827 WB Image

WB analysis of HT-29 (lanes 1 and 3) and Colo-205 (lanes 2 and 4) cell line lysates using GTX54827 Thrombin Receptor antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-Thrombin Receptor antibody used in Western Blot (WB). GTX54827

GTX54827 WB Image

WB analysis of HL-60 (lanes 1 and 4), Jurkat (lanes 2 and 5), and K562 (lanes 3 and 6) cell line lysates using GTX54827 Thrombin Receptor antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-Thrombin Receptor antibody used in Live cell imaging (LCI). GTX54827

GTX54827 LCI Image

Live cell imaging analysis of PC-3 cells using GTX54827 Thrombin Receptor antibody.
Red : Primary antibody
Blue : Hoechst 33342 for nuclear staining
Dilution : 1:50

Anti-Thrombin Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX54827

GTX54827 IHC-P Image

IHC-P analysis of normal human breast and human breast carcinoma tissue using GTX54827 Thrombin Receptor antibody.  PAR-1 staining is highly specific for epithelium-derived cells.
Panel A : In the normal resting breast, epithelial cells of the mammary ducts are visible using Histofine (pink)
Panel B :  The breast carcinoma contains epithelium-derived malignant cells stained with DAB (brown).  Hematoxilin is used as the counterstain.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639