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Thrombin antibody

Anti-Thrombin antibody used in Western Blot (WB). GTX101270
Anti-Thrombin antibody used in Western Blot (WB). GTX101270
Anti-Thrombin antibody used in Western Blot (WB). GTX101270
Anti-Thrombin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101270

Cat. No. GTX101270

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Thrombin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Thrombin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950244

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coagulation factor II, thrombin , PT , RPRGL2 , THPH1

Cellular Localization

Secreted , extracellular space

Background

Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Thrombin antibody used in Western Blot (WB). GTX101270

GTX101270 WB Image

Human plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Thrombin antibody (GTX101270) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Thrombin antibody used in Western Blot (WB). GTX101270

GTX101270 WB Image

Mouse plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Thrombin antibody (GTX101270) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Thrombin antibody used in Western Blot (WB). GTX101270

GTX101270 WB Image

Rat plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Thrombin antibody (GTX101270) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Thrombin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101270

GTX101270 ICC/IF Image

Thrombin antibody detects Thrombin protein by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: Thrombin stained by Thrombin antibody (GTX101270) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Yang F et al. Cancer Res 2020; 80 (18) : 3892-3905 Interaction with CD68 and Regulation of GAS6 Expression by Endosialin in Fibroblasts Drives Recruitment and Polarization of Macrophages in Hepatocellular Carcinoma.
Application : WB
Reactivity : Human
Lin SW et al. J Infect 2012; 64 (3) : 325-34 Dengue virus nonstructural protein NS1 binds to prothrombin/thrombin and inhibits prothrombin activation.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169