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Thrombin antibody

Anti-Thrombin antibody used in Western Blot (WB). GTX101270
Anti-Thrombin antibody used in Western Blot (WB). GTX101270
Anti-Thrombin antibody used in Western Blot (WB). GTX101270

Cat No. GTX101270

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Target recombinant protein

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Thrombin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

coagulation factor II, thrombin , PT , RPRGL2 , THPH1

Cellular Localization

Secreted , extracellular space

Background

Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Thrombin antibody used in Western Blot (WB). GTX101270

GTX101270 WB Image

Human plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Thrombin antibody (GTX101270) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Thrombin antibody used in Western Blot (WB). GTX101270

GTX101270 WB Image

Mouse plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Thrombin antibody (GTX101270) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Thrombin antibody used in Western Blot (WB). GTX101270

GTX101270 WB Image

Rat plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Thrombin antibody (GTX101270) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169