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Thrombomodulin antibody [C3], C-term

Orthogonal
Anti-Thrombomodulin antibody [C3], C-term used in Western Blot (WB). GTX108277
Anti-Thrombomodulin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108277
Anti-Thrombomodulin antibody [C3], C-term used in Western Blot (WB). GTX108277
Anti-Thrombomodulin antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108277
Anti-Thrombomodulin antibody [C3], C-term used in Immunoprecipitation (IP). GTX108277

Cat. No. GTX108277

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Thrombomodulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human Thrombomodulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952233

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thrombomodulin , AHUS6 , BDCA3 , CD141 , THPH12 , THRM , TM

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this intronless gene is an endothelial-specific type I membrane receptor that binds thrombin. This binding results in the activation of protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Thrombomodulin antibody [C3], C-term used in Western Blot (WB). GTX108277

GTX108277 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Thrombomodulin antibody [C3], C-term (GTX108277) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Thrombomodulin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108277

GTX108277 IHC-P Image

Thrombomodulin antibody [C3], C-term detects THBD protein at cytosol and membrane on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
Thrombomodulin antibody [C3], C-term (GTX108277) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Thrombomodulin antibody [C3], C-term used in Western Blot (WB). GTX108277

GTX108277 WB Image

Thrombomodulin antibody detects Thrombomodulin protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Thrombomodulin antibody (GTX108277) diluted by 1:500.

Anti-Thrombomodulin antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108277

GTX108277 ICC/IF Image

Thrombomodulin antibody [C3], C-term detects Thrombomodulin protein at junction by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Thrombomodulin protein stained by Thrombomodulin antibody [C3], C-term (GTX108277) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-Thrombomodulin antibody [C3], C-term used in Immunoprecipitation (IP). GTX108277

GTX108277 IP Image

Immunoprecipitation of Thrombomodulin protein from THP-1 whole cell extracts using 5 μg of Thrombomodulin antibody [C3], C-term (GTX108277).
Western blot analysis was performed using Thrombomodulin antibody [C3], C-term (GTX108277).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169