GeneTex
United States (US)

Thrombomodulin antibody [C3], C-term

GTX108277_39757_WB_19011507_322.jpg
GTX108277_41766_20150629_IFA_19011507_466.jpg
GTX108277_39757_20151204_IP_19011507_561.jpg
GTX108277_39757_IHC_19011507_166.jpg

Cat No. GTX108277

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

THP-1
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Thrombomodulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Thrombomodulin,Ahus6,Bdca3,Cd141,Thph12,Thrm,Tm,Thbd

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this intronless gene is an endothelial-specific type I membrane receptor that binds thrombin. This binding results in the activation of protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108277_39757_WB_19011507_322.jpg

GTX108277 WB Image

Sample (30 µg of whole cell lysate)
A: THP-1
7.5% SDS PAGE
GTX108277 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108277_41766_20150629_IFA_19011507_466.jpg

GTX108277 ICC/IF Image

Thrombomodulin antibody [C3], C-term detects Thrombomodulin protein at junction by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Thrombomodulin protein stained by Thrombomodulin antibody [C3], C-term (GTX108277) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX108277_39757_20151204_IP_19011507_561.jpg

GTX108277 IP Image

Immunoprecipitation of Thrombomodulin protein from THP-1 whole cell extracts using 5 μg of Thrombomodulin antibody [C3], C-term (GTX108277).
Western blot analysis was performed using Thrombomodulin antibody [C3], C-term (GTX108277).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX108277_39757_IHC_19011507_166.jpg

GTX108277 IHC-P Image

Thrombomodulin antibody [C3], C-term detects THBD protein at cytosol and membrane on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
Thrombomodulin antibody [C3], C-term (GTX108277) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf