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Thrombospondin 2 antibody [HL1140]

Anti-Thrombospondin 2 antibody [HL1140] used in Western Blot (WB). GTX636411
Anti-Thrombospondin 2 antibody [HL1140] used in ELISA (ELISA). GTX636411
Anti-Thrombospondin 2 antibody [HL1140] used in IHC (Paraffin sections) (IHC-P). GTX636411
Anti-Thrombospondin 2 antibody [HL1140] used in Western Blot (WB). GTX636411
Anti-Thrombospondin 2 antibody [HL1140] used in ELISA (ELISA). GTX636411
Anti-Thrombospondin 2 antibody [HL1140] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636411

Cat. No. GTX636411

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1140

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA, Sandwich ELISA

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX636506, Detection : GTX636411 or Capture : GTX636411, Detection : GTX636506. Please notice that the detected antibody needs to be conjugated to HRP when paired with the capture antibody. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Calculated MW

130 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Thrombospondin 2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909977

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thrombospondin 2 , TSP2

Background

The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Thrombospondin 2 antibody [HL1140] used in Western Blot (WB). GTX636411

GTX636411 WB Image

AsPC-1 whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1140] (GTX636411) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Thrombospondin 2 antibody [HL1140] used in ELISA (ELISA). GTX636411

GTX636411 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length human thrombospondin 2 protein using antibodies as below.

Capture: Thrombospondin 2 antibody [HL1140] (GTX636411) (5 μg/mL)
Detection: HRP-conjugated Thrombospondin 2 antibody [HL1194] (GTX636506) (1 μg/mL)
Please notice that GTX636506 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636411. Please contact us for custom HRP-conjugated antibody.

Anti-Thrombospondin 2 antibody [HL1140] used in IHC (Paraffin sections) (IHC-P). GTX636411

GTX636411 IHC-P Image

Thrombospondin 2 antibody [HL1140] detects Thrombospondin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
Thrombospondin 2 stained by Thrombospondin 2 antibody [HL1140] (GTX636411) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Thrombospondin 2 antibody [HL1140] used in Western Blot (WB). GTX636411

GTX636411 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1140] (GTX636411) diluted at 1:300000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Thrombospondin 2 antibody [HL1140] used in ELISA (ELISA). GTX636411

GTX636411 ELISA Image

Sandwich ELISA detection of recombinant human thrombospondin 2 protein using antibodies as below.

Capture: Thrombospondin 2 antibody [HL1194] (GTX636506) (5 μg/mL)
Detection: HRP-conjugated Thrombospondin 2 antibody [HL1140] (GTX636411) (1 μg/mL)
Please notice that GTX636411 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636506. Please contact us for custom HRP-conjugated antibody.

Anti-Thrombospondin 2 antibody [HL1140] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636411

GTX636411 ICC/IF Image

Thrombospondin 2 antibody [HL1140] detects Thrombospondin 2 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Thrombospondin 2 stained by Thrombospondin 2 antibody [HL1140] (GTX636411) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

There are currently no references for Thrombospondin 2 antibody [HL1140] (GTX636411). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations