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Thrombospondin 2 antibody

Orthogonal Overexpression
Anti-Thrombospondin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX134554
Anti-Thrombospondin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX134554
Anti-Thrombospondin 2 antibody used in Western Blot (WB). GTX134554
Anti-Thrombospondin 2 antibody used in Western Blot (WB). GTX134554

Cat. No. GTX134554

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Thrombospondin 2 products
PRODUCT

Summary

Thrombospondin 2, also known as THBS2 and TSP2, is a secreted glycoprotein with a predicted molecular weight of 130 kDa. THBS2 mediates cell-to-cell and cell-to-matrix interactions, and can be expressed together with CA 19-9 in patients with early pancreatic ductal adenocarcinoma.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

130 kDa. ( Note )

Positive Control

AsPC-1 conditioned medium

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Thrombospondin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.
Glycerol|PBS|ProClin

Conjugation

Unconjugated

RRID

AB_2887309

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thrombospondin 2 , TSP2

Background

The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Thrombospondin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX134554

GTX134554 IHC-P Image

Thrombospondin 2 antibody detects Thrombospondin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
Thrombospondin 2 stained by Thrombospondin 2 antibody (GTX134554) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Thrombospondin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX134554

GTX134554 IHC-P Image

Thrombospondin 2 antibody detects Thrombospondin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
Thrombospondin 2 stained by Thrombospondin 2 antibody (GTX134554) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Thrombospondin 2 antibody used in Western Blot (WB). GTX134554

GTX134554 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody (GTX134554) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Thrombospondin 2 antibody used in Western Blot (WB). GTX134554

GTX134554 WB Image

Orthogonal

AsPC-1 whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody (GTX134554) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169