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Thrombospondin 2 antibody

Orthogonal
Anti-Thrombospondin 2 antibody used in Western Blot (WB). GTX134576
Anti-Thrombospondin 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134576
Anti-Thrombospondin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX134576

Cat. No. GTX134576

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Thrombospondin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

130 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Thrombospondin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887312

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

thrombospondin 2 , TSP2

Background

The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Thrombospondin 2 antibody used in Western Blot (WB). GTX134576

GTX134576 WB Image

Orthogonal

AsPC-1 whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody (GTX134576) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 29242498

Anti-Thrombospondin 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134576

GTX134576 ICC/IF Image

Thrombospondin 2 antibody detects Thrombospondin 2 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Thrombospondin 2 stained by Thrombospondin 2 antibody (GTX134576) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Thrombospondin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX134576

GTX134576 IHC-P Image

Thrombospondin 2 antibody detects Thrombospondin 2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Thrombospondin 2 stained by Thrombospondin 2 antibody (GTX134576) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169