Wild-type (WT) and Thymidine Kinase 1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Thymidine Kinase 1 antibody (GTX113281) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemical analysis of paraffin-embedded mouse muscle, using Thymidine Kinase 1 (GTX113281) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

A431 whole cell and cytoplasm extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Thymidine Kinase 1 antibody (GTX113281) diluted at 1:1000.

Thymidine Kinase 1 antibody detects Thymidine Kinase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
Thymidine Kinase 1 stained by Thymidine Kinase 1 antibody (GTX113281) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Thymidine Kinase 1 (GTX113281) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Thymidine Kinase 1 antibody (GTX113281) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Mol Imaging Biol in 2017. PMID: 27798786