GeneTex

  • Country / Location Selection

United States (US)

Thymidine Kinase 1 antibody

KO/KD Orthogonal
Anti-Thymidine Kinase 1 antibody used in Western Blot (WB). GTX113281
Anti-Thymidine Kinase 1 antibody used in Western Blot (WB). GTX113281
Anti-Thymidine Kinase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113281
Anti-Thymidine Kinase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113281
Anti-Thymidine Kinase 1 antibody used in Western Blot (WB). GTX113281
Anti-Thymidine Kinase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113281
Anti-Thymidine Kinase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113281

Cat. No. GTX113281

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Thymidine Kinase 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

A431 , 293T

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Thymidine Kinase 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11178067

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thymidine kinase 1 , TK2

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-Thymidine Kinase 1 antibody used in Western Blot (WB). GTX113281

GTX113281 WB Image

KO/KD

Wild-type (WT) and Thymidine Kinase 1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Thymidine Kinase 1 antibody (GTX113281) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Thymidine Kinase 1 antibody used in Western Blot (WB). GTX113281

GTX113281 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Thymidine Kinase 1 antibody (GTX113281) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Thymidine Kinase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113281

GTX113281 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Thymidine Kinase 1 (GTX113281) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Thymidine Kinase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113281

GTX113281 IHC-P Image

Thymidine Kinase 1 antibody detects Thymidine Kinase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
Thymidine Kinase 1 stained by Thymidine Kinase 1 antibody (GTX113281) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Thymidine Kinase 1 antibody used in Western Blot (WB). GTX113281

GTX113281 WB Image

Orthogonal

A431 whole cell and cytoplasm extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Thymidine Kinase 1 antibody (GTX113281) diluted at 1:1000.

Anti-Thymidine Kinase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113281

GTX113281 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse muscle, using Thymidine Kinase 1 (GTX113281) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Thymidine Kinase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113281

GTX113281 IHC-P Image

The data was published in the journal Mol Imaging Biol in 2017. PMID: 27798786

REFERENCE

Application Reference

Gandhi R et al. J Nucl Cardiol 2021; 28 (5) : 1961-1971 Cell proliferation detected using [18F]FLT PET/CT as an early marker of abdominal aortic aneurysm.
Cho CJ et al. Oncol Lett 2019; 18 (6) : 6869-6876 The modulation study of multiple drug resistance in bladder cancer by curcumin and resveratrol.
Heskamp S et al. Mol Imaging Biol 2017; 19 (4) : 540-549 Response Monitoring with [18F]FLT PET and Diffusion-Weighted MRI After Cytotoxic 5-FU Treatment in an Experimental Rat Model for Colorectal Liver Metastases.
Application : IHC-P
Reactivity : Rat
Kuo WT et al. Oncol Rep 2017; (Epub) High * sensitivity of docetaxel-resistant A549 cells is mediated by TP53 status and downregulated thymidylate synthase.
Application : WB
Submit a Reference

REVIEW

There are currently no reviews for Thymidine Kinase 1 antibody (GTX113281). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169