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Thymidine Kinase 1 antibody

Anti-Thymidine Kinase 1 antibody used in Western Blot (WB). GTX33544
Anti-Thymidine Kinase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33544

Cat No. GTX33544

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-234 of human TK1 (NP_003249.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

thymidine kinase 1 , TK2

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers. [provided by RefSeq, Oct 2016]

Database

Research Area

DATA IMAGES

Anti-Thymidine Kinase 1 antibody used in Western Blot (WB). GTX33544

GTX33544 WB Image

WB analysis of various sample lysates using GTX33544 Thymidine Kinase 1 antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-Thymidine Kinase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33544

GTX33544 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33544 Thymidine Kinase 1 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329