Thymidylate synthase antibody [AT1S5]

Cat. No. GTX57572

Host	Mouse
Clonality	Monoclonal
Clone Name	AT1S5
Isotype	IgG2b
Application	WB, ICC/IF, FACS
Reactivity	Human
Package	100 μl ($399)

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Datasheet
Document

View product citations for antibody GTX57572 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:1000
ICC/IF	1:500-1:1000
FACS	Assay dependent

Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT1S5 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TYMS protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thymidylate synthetase , HST422 , TMS , TS , TYMS , Thymidylate Synthase

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion,Mitochondrion matrix,Mitochondrion inner membrane

Background

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy. [provided by RefSeq, Aug 2017]

Database

Gene ID: 7298 TYMS
UniProt: P04818 TYMS

Research Area

DATA IMAGES

GTX57572 WB Image

WB analysis of various samples using GTX57572 Thymidylate synthase antibody.
Lane 1 : Recombinant Human Thymidylate synthase protein (50 ng)
Lane 2 : HeLa whole cell lysate (40 μg)
Lane 3 : Jurkat whole cell lysate (40 μg)
Lane 4 : MCF-7 whole cell lysate (40 μg)

GTX57572 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57572 Thymidylate synthase antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

GTX57572 FACS Image

FACS analysis of HeLa cells using GTX57572 Thymidylate synthase antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty