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Thymidylate synthase antibody [HL1236]

Recombinant antibody
Anti-Thymidylate synthase antibody [HL1236] used in Western Blot (WB). GTX636635
Anti-Thymidylate synthase antibody [HL1236] used in IHC (Paraffin sections) (IHC-P). GTX636635
Anti-Thymidylate synthase antibody [HL1236] used in IHC (Paraffin sections) (IHC-P). GTX636635
Anti-Thymidylate synthase antibody [HL1236] used in IHC (Paraffin sections) (IHC-P). GTX636635
Anti-Thymidylate synthase antibody [HL1236] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636635
Anti-Thymidylate synthase antibody [HL1236] used in Western Blot (WB). GTX636635
Anti-Thymidylate synthase antibody [HL1236] used in Western Blot (WB). GTX636635

Cat. No. GTX636635

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1236

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Thymidylate synthase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Predict Reactivity

Zebrafish, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Thymidylate synthase. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thymidylate synthetase , HST422 , TMS , TS

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion,Mitochondrion matrix,Mitochondrion inner membrane

Background

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Thymidylate synthase antibody [HL1236] used in Western Blot (WB). GTX636635

GTX636635 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (GTX636635) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Thymidylate synthase antibody [HL1236] used in IHC (Paraffin sections) (IHC-P). GTX636635

GTX636635 IHC-P Image

Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog tissue.
Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (GTX636635) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Thymidylate synthase antibody [HL1236] used in IHC (Paraffin sections) (IHC-P). GTX636635

GTX636635 IHC-P Image

Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat brain.
Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (GTX636635) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Thymidylate synthase antibody [HL1236] used in IHC (Paraffin sections) (IHC-P). GTX636635

GTX636635 IHC-P Image

Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog brain.
Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (GTX636635) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Thymidylate synthase antibody [HL1236] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636635

GTX636635 ICC/IF Image

Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cytoplasm and nucleus and mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (GTX636635) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Thymidylate synthase antibody [HL1236] used in Western Blot (WB). GTX636635

GTX636635 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (GTX636635) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Thymidylate synthase antibody [HL1236] used in Western Blot (WB). GTX636635

GTX636635 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (GTX636635) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations