APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Zebrafish, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Thymidylate synthase. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2910019
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
thymidylate synthetase , HST422 , TMS , TS
Cellular Localization
Nucleus,Cytoplasm,Mitochondrion,Mitochondrion matrix,Mitochondrion inner membrane
Background
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
![Anti-Thymidylate synthase antibody [HL1237] used in Western Blot (WB). GTX636636](/templates/default/js/lightbox/images/loading.gif "Anti-Thymidylate synthase antibody [HL1237] used in Western Blot (WB). GTX636636")
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GTX636636 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1237] (GTX636636) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636636 IHC-P Image
Thymidylate synthase antibody [HL1237] detects Thymidylate synthase protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse thymus gland.Thymidylate synthase stained by Thymidylate synthase antibody [HL1237] (GTX636636) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636636 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1237] (GTX636636) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636636 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1237] (GTX636636) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for Thymidylate synthase antibody [HL1237] (GTX636636). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Thymidylate synthase antibody [HL1237] (GTX636636). Be the first to share your experience with this product.
![Anti-Thymidylate synthase antibody [HL1237] used in Western Blot (WB). GTX636636](/upload/website/prouct_img/small/GTX636636/GTX636636_44620_20221216_WB_D_C_22122018_936.webp "Anti-Thymidylate synthase antibody [HL1237] used in Western Blot (WB). GTX636636")
![Anti-Thymidylate synthase antibody [HL1237] used in IHC (Paraffin sections) (IHC-P). GTX636636](/upload/website/prouct_img/small/GTX636636/GTX636636_T-44515_20220506_IHC-P_M_w_23061202_493.webp "Anti-Thymidylate synthase antibody [HL1237] used in IHC (Paraffin sections) (IHC-P). GTX636636")
![Anti-Thymidylate synthase antibody [HL1237] used in Western Blot (WB). GTX636636](/upload/website/prouct_img/small/GTX636636/GTX636636_44620_20220318_WB_w_23061202_541.webp "Anti-Thymidylate synthase antibody [HL1237] used in Western Blot (WB). GTX636636")
![Anti-Thymidylate synthase antibody [HL1237] used in Western Blot (WB). GTX636636](/upload/website/prouct_img/small/GTX636636/GTX636636_44620_20230818_WB_Drosophila_23082201_375.webp "Anti-Thymidylate synthase antibody [HL1237] used in Western Blot (WB). GTX636636")
![Anti-Thymidylate synthase antibody [HL1237] used in Western Blot (WB). GTX636636](/upload/website/prouct_img/normal/GTX636636/GTX636636_44620_20221216_WB_D_C_22122018_936.webp "Anti-Thymidylate synthase antibody [HL1237] used in Western Blot (WB). GTX636636")
![Anti-Thymidylate synthase antibody [HL1237] used in IHC (Paraffin sections) (IHC-P). GTX636636](/upload/website/prouct_img/normal/GTX636636/GTX636636_T-44515_20220506_IHC-P_M_w_23061202_493.webp "Anti-Thymidylate synthase antibody [HL1237] used in IHC (Paraffin sections) (IHC-P). GTX636636")
![Anti-Thymidylate synthase antibody [HL1237] used in Western Blot (WB). GTX636636](/upload/website/prouct_img/normal/GTX636636/GTX636636_44620_20220318_WB_w_23061202_541.webp "Anti-Thymidylate synthase antibody [HL1237] used in Western Blot (WB). GTX636636")
![Anti-Thymidylate synthase antibody [HL1237] used in Western Blot (WB). GTX636636](/upload/website/prouct_img/normal/GTX636636/GTX636636_44620_20230818_WB_Drosophila_23082201_375.webp "Anti-Thymidylate synthase antibody [HL1237] used in Western Blot (WB). GTX636636")