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Thymidylate synthase antibody [N3C3]

Anti-Thymidylate synthase antibody [N3C3] used in Western Blot (WB). GTX113289
Anti-Thymidylate synthase antibody [N3C3] used in Western Blot (WB). GTX113289

Cat. No. GTX113289

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Thymidylate synthase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Dog, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Thymidylate synthase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038196

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thymidylate synthetase , HST422 , TMS , TS

Background

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Thymidylate synthase antibody [N3C3] used in Western Blot (WB). GTX113289

GTX113289 WB Image

Zebrafish tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Thymidylate synthetase antibody [N3C3] (GTX113289) diluted at 1:500.

Anti-Thymidylate synthase antibody [N3C3] used in Western Blot (WB). GTX113289

GTX113289 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Thymidylate synthetase antibody [N3C3] (GTX113289) diluted at 1:1000.

REFERENCE

Application Reference

Hsin IL et al. Cancers (Basel) 2019; 12 (1) The Application of Arsenic Trioxide in Ameliorating ABT-737 Target Therapy on Uterine Cervical Cancer Cells through Unique Pathways in Cell Death.
Application : WB
Reactivity : Human
Ko JL et al. Environ Toxicol 2018; (Epub) Effects and mechanisms of betulinic acid on improving EGFR TKI-resistance of lung cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169