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Thymidylate synthase antibody [SPM453]

Anti-Thymidylate synthase antibody [SPM453] used in IHC (Paraffin sections) (IHC-P). GTX35113

Cat. No. GTX35113

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM453

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($529)
See all Thymidylate synthase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human TYMS protein.

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HST422 , TMS , TS , TYMS , thymidylate synthetase , Thymidylate Synthase

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion,Mitochondrion matrix,Mitochondrion inner membrane

Background

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Thymidylate synthase antibody [SPM453] used in IHC (Paraffin sections) (IHC-P). GTX35113

GTX35113 IHC-P Image

IHC-P analysis of human testicular carcinoma tissue using GTX35113 Thymidylate synthase antibody [SPM453].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529